|
|
Registro Completo |
Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
25/10/2017 |
Data da última atualização: |
06/05/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MOURA, C. J. M. de; FAJARDO, T. V. M.; EIRAS, M.; SILVA, F. N. da; NICKEL, O. |
Afiliação: |
Cátia Jacira Martins de Moura, 1Biological Institute ? Dept. of Plant Pathology ? Lab. of Plant Virology, Av. Conselheiro Rodrigues Alves, 1252 ? 04014-900 ? São Paulo, SP ? Brazil.; THOR VINICIUS MARTINS FAJARDO, CNPUV; Marcelo Eiras, 1Biological Institute ? Dept. of Plant Pathology ? Lab. of Plant Virology, Av. Conselheiro Rodrigues Alves, 1252 ? 04014-900 ? São Paulo, SP ? Brazil.; Fábio Nascimento da Silva, 3Santa Catarina State University/Centre of Agroveterinary Sciences, Av. Luiz de Camões, 2090 ? 88520-000 ? Lages, SC ? Brazil.; OSMAR NICKEL, CNPUV. |
Título: |
Molecular characterization of GSyV-1 and GLRaV-3 and prevalence of grapevine viruses in a grape-growing area. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Scientia Agricola, Brasília,DF, v. 75, n. 1, p.43-51, jan./feb. 2018. |
DOI: |
10.1590/1678-992X-2016-0328 |
Idioma: |
Inglês |
Conteúdo: |
The aims of this study were to determine the prevalence of viruses in 119 samples from 32 grapevine cultivars, collected from nine vineyards in a specific grape-growing area in southeastern Brazil, perform a partial molecular characterization of 14 isolates of Grapevine Syrah virus 1 (GSyV-1) and Grapevine leafroll-associated virus 3 (GLRaV-3) and assess the coat protein genetic variability of these viruses. The detection of viruses was implemented by realtime RT-PCR (reverse transcription polymerase chain reaction) aiming to detect seven viruses and one viroid. With the exception of the Grapevine Cabernet Sauvignon reovirus (GCSV), the viruses and viroid that were evaluated were widespread in the sampled areas, often in high prevalence and multiple infections, ranging from 15 % up to 76 %. Eight isolates of GSyV-1 and six of GLRaV-3, partially characterized by complete coat protein gene nucleotide sequencing and a variability study showed nucleotide identities ranging from 91 % to 99 % (GSyV-1) and from 98 % to 100 % (GLRaV-3) among themselves, respectively. Comparisons between conventional and real-time RT-PCR detections were implemented for GSyV-1 and GLRaV-3 infections. Analysis of genetic variability indicated molecular differences between GSyV-1 and GLRaV-3 isolates and negative selection acting on the coat protein gene of both viruses. This is the first report of GSyV-1 in commercial vineyards in Brazil. The survey revealed widespread infections of seven important pathogens in one prominent Brazilian grape-producing region implying contaminated grapevine cuttings in the spread of disease. Keywords: Vitis, diagnosis, variability, incidence, leafroll MenosThe aims of this study were to determine the prevalence of viruses in 119 samples from 32 grapevine cultivars, collected from nine vineyards in a specific grape-growing area in southeastern Brazil, perform a partial molecular characterization of 14 isolates of Grapevine Syrah virus 1 (GSyV-1) and Grapevine leafroll-associated virus 3 (GLRaV-3) and assess the coat protein genetic variability of these viruses. The detection of viruses was implemented by realtime RT-PCR (reverse transcription polymerase chain reaction) aiming to detect seven viruses and one viroid. With the exception of the Grapevine Cabernet Sauvignon reovirus (GCSV), the viruses and viroid that were evaluated were widespread in the sampled areas, often in high prevalence and multiple infections, ranging from 15 % up to 76 %. Eight isolates of GSyV-1 and six of GLRaV-3, partially characterized by complete coat protein gene nucleotide sequencing and a variability study showed nucleotide identities ranging from 91 % to 99 % (GSyV-1) and from 98 % to 100 % (GLRaV-3) among themselves, respectively. Comparisons between conventional and real-time RT-PCR detections were implemented for GSyV-1 and GLRaV-3 infections. Analysis of genetic variability indicated molecular differences between GSyV-1 and GLRaV-3 isolates and negative selection acting on the coat protein gene of both viruses. This is the first report of GSyV-1 in commercial vineyards in Brazil. The survey revealed widespread infections of seven important pat... Mostrar Tudo |
Palavras-Chave: |
Caracterização molecular; Cultivo de uva; Diagnosis; Leafroll; Variabilitty; Videira; Vírus em videira. |
Thesagro: |
DOença de planta; Uva. |
Thesaurus Nal: |
incidence; Vitis. |
Categoria do assunto: |
A Sistemas de Cultivo |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/165554/1/SA-Molecular-characterization-GSyV1-and-GLRaV3-2018.pdf
|
Marc: |
LEADER 02596naa a2200313 a 4500 001 2078147 005 2019-05-06 008 2018 bl uuuu u00u1 u #d 024 7 $a10.1590/1678-992X-2016-0328$2DOI 100 1 $aMOURA, C. J. M. de 245 $aMolecular characterization of GSyV-1 and GLRaV-3 and prevalence of grapevine viruses in a grape-growing area.$h[electronic resource] 260 $c2018 520 $aThe aims of this study were to determine the prevalence of viruses in 119 samples from 32 grapevine cultivars, collected from nine vineyards in a specific grape-growing area in southeastern Brazil, perform a partial molecular characterization of 14 isolates of Grapevine Syrah virus 1 (GSyV-1) and Grapevine leafroll-associated virus 3 (GLRaV-3) and assess the coat protein genetic variability of these viruses. The detection of viruses was implemented by realtime RT-PCR (reverse transcription polymerase chain reaction) aiming to detect seven viruses and one viroid. With the exception of the Grapevine Cabernet Sauvignon reovirus (GCSV), the viruses and viroid that were evaluated were widespread in the sampled areas, often in high prevalence and multiple infections, ranging from 15 % up to 76 %. Eight isolates of GSyV-1 and six of GLRaV-3, partially characterized by complete coat protein gene nucleotide sequencing and a variability study showed nucleotide identities ranging from 91 % to 99 % (GSyV-1) and from 98 % to 100 % (GLRaV-3) among themselves, respectively. Comparisons between conventional and real-time RT-PCR detections were implemented for GSyV-1 and GLRaV-3 infections. Analysis of genetic variability indicated molecular differences between GSyV-1 and GLRaV-3 isolates and negative selection acting on the coat protein gene of both viruses. This is the first report of GSyV-1 in commercial vineyards in Brazil. The survey revealed widespread infections of seven important pathogens in one prominent Brazilian grape-producing region implying contaminated grapevine cuttings in the spread of disease. Keywords: Vitis, diagnosis, variability, incidence, leafroll 650 $aincidence 650 $aVitis 650 $aDOença de planta 650 $aUva 653 $aCaracterização molecular 653 $aCultivo de uva 653 $aDiagnosis 653 $aLeafroll 653 $aVariabilitty 653 $aVideira 653 $aVírus em videira 700 1 $aFAJARDO, T. V. M. 700 1 $aEIRAS, M. 700 1 $aSILVA, F. N. da 700 1 $aNICKEL, O. 773 $tScientia Agricola, Brasília,DF$gv. 75, n. 1, p.43-51, jan./feb. 2018.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Uva e Vinho (CNPUV) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
05/12/2012 |
Data da última atualização: |
23/10/2014 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
FERNANDES, S. G.; MACHADO, C. T. de T.; IGNACIO, I. G.; PEREIRA, C. D.; FERNANDES, L. A. |
Afiliação: |
MESTRE EM CIÊNCIAS AGRÁRIA; CYNTHIA TORRES DE TOLEDO MACHADO, CPAC; ITAMAR GARCIA IGNACIO, CNPAB; CICERO DONIZETE PEREIRA, CPAC; CAMPUS REGIONAL DE MONTES CLAROS. |
Título: |
Atividade micorrízica em áreas de agricultores familiares no Norte de Minas Gerais. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
In: REUNIÃO BRASILEIRA DE CIÊNCIA DO SOLO E NUTRIÇÃO DE PLANTAS, 30.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 14.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 12.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 9.; SIMPÓSIO SOBRE SELÊNIO NO BRASIL, 1., 2012, Maceió. A responsabilidade socioambiental da pesquisa agrícola: anais. Viçosa, MG: SBCS, 2012. FERTBIO 2012. |
Idioma: |
Português |
Palavras-Chave: |
Potencial de anóculo; Práticas agroecológicas; Simbiose micorrízica. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00901nam a2200181 a 4500 001 1941346 005 2014-10-23 008 2012 bl uuuu u01u1 u #d 100 1 $aFERNANDES, S. G. 245 $aAtividade micorrízica em áreas de agricultores familiares no Norte de Minas Gerais. 260 $aIn: REUNIÃO BRASILEIRA DE CIÊNCIA DO SOLO E NUTRIÇÃO DE PLANTAS, 30.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 14.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 12.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 9.; SIMPÓSIO SOBRE SELÊNIO NO BRASIL, 1., 2012, Maceió. A responsabilidade socioambiental da pesquisa agrícola: anais. Viçosa, MG: SBCS, 2012. FERTBIO 2012.$c2012 653 $aPotencial de anóculo 653 $aPráticas agroecológicas 653 $aSimbiose micorrízica 700 1 $aMACHADO, C. T. de T. 700 1 $aIGNACIO, I. G. 700 1 $aPEREIRA, C. D. 700 1 $aFERNANDES, L. A.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Agrobiologia (CNPAB) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|